Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro Interleukin 1 beta), IgG2a, Clone: [8B25], Rat, Monoclonal10

Product Overview

• Article Name: Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro Interleukin 1 beta), IgG2a, Clone: [8B25], Rat, Monoclonal10
• Biozol Catalog Number: USB-I7663-20A
• Supplier Catalog Number: I7663-20A
• Alternative Catalog Number: USB-I7663-20A-100
• Manufacturer: US Biological
• Host: Rat
• Category: Antikörper
• Application: ELISA, WB
• Immunogen: Recombinant protein corresponding to Ala116-Ala268 of equine IL-1 beta expressed in E. coli (Q28386). Species Sequence Homology: 64-72% aa sequence identity with bovine, feline, goat, human, mouse, sheep, porcine, rabbit and rat IL-1beta.

Product Description

IL-1beta, also known as IL1F2, is a prototypical member of the IL-1 superfamily. It is produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. The biological activity of IL-1beta is mediated by the heterodimeric receptor complex containing IL-1 R1 (IL-1 RI) and IL-1 R3 (IL-1 RAcP). The IL-1beta propeptide is cleaved intracellularly by caspase-1/ICE to generate a 17kD active cytokine. An alternatively spliced form of equine IL-1beta lacks the caspase-1 cleavage site and is potentially membrane-associated. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit for recombinant equine IL-1b is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5 to 50 fold Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant equine IL-1b is ~2ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [8B25]
• Isotype: IgG2a
• NCBI: 001075995
• UniProt: Q28386
• Purity: Purified by Protein G affinity chromatography from culture supernatant.
• Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.