Recombinant mouse Interleukin-1 beta (IL-1b), expressed in E. coli
Applications: Suitable for use in Neutralization, Sandwich ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Sandwich ELISA: 0.5-2.0ug/ml using 100ul/well,. The detection is ~2000-4000pg/ml of recombinant mouse Interleukin 1 beta (IL1b). Western Blot: 0.1-0.2ug/ml. Detects1.5-3.0ng/lane, under either reducing or non-reducing conditions. Neutralization: 0.57-0.86ug/ml Yields one-half maximal inhibition [ND50] of the biological activity (10pg/ml)). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
Purity:
Purified by immunoaffinity chromatography
Form:
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O.
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