IL-10 Rbeta (Interleukin 10 receptor beta, also IL-10R2, CD210b and CRF2-4) is an 80-85kD member of the type II cytokine receptor family of proteins. It is very widely expressed, and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B and -29 (plus IL-26 in human). Mature mouse IL-10 Rbeta is a type I transmembrane protein that is 330 amino acids (aa) in length. It contains a 201 aa extracellular domain (aa 20-220) that possesses two fibronectin type III domains (aa 22-107 and 111-208). There is one alternate start site at Met20. Over aa 21-220, mouse IL-10 Rbeta shares 87% and 74% aa identity with rat and human IL-10 Rbeta, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit for rmIL-10 Rbeta is approximately 1ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
