Recombinant protein corresponding to Met20-Ser220 of human Interleukin 10 receptor beta expressed in NS0 cells.
The IL-10 receptor consists of two chains, a ligand binding IL-10 Ra (IL-10 R1) chain and a signaling chain known as IL-10 Rb (IL-10 R2).1, 2 The human IL-10 Rb chain is a 325aa protein with 69% homology to its mouse counterpart.3 Stimulation of cells by IL-10 activates both Jak1 and Tyk2 kinases.2 The IL-10 Rb chain is also a component of the recently identified IL-22 receptor.4 Both chains of the IL-10 R are expressed on lymphocytes, monocytes, and neutrophils, with the IL-10 Rb chain expressed at higher levels than the IL-10 Ra chain in most cases.5 IL-10 signaling can affect a variety of immune functions, including inhibition of pro-inflammatory cytokine synthesis and regulation of the growth and function of B cells and antigen-presenting cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 human peripheral blood monocytes Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells Balb/c mice. Absorption: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
