Interleukin 15 (IL-15) (Biotin), Rabbit
Catalog Number:
USB-I8437-03
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| Article Name: | Interleukin 15 (IL-15) (Biotin), Rabbit |
| Biozol Catalog Number: | USB-I8437-03 |
| Supplier Catalog Number: | I8437-03 |
| Alternative Catalog Number: | USB-I8437-03-25,USB-I8437-03-50 |
| Manufacturer: | US Biological |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Immunogen: | Recombinant protein corresponding to human IL-15, expressed in E. coli. |
| IL-15 is a member of the four helix-bundle cytokine family with a molecular weight of 14-15 kD. Its in vitro activity is very similar to that of IL2, except that IL-15 utilizes the IL-15 receptor alpha subunit for binding. IL15 play an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes. It also activates a number of importatnat intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (detection): 0.25-1ug/ml (100ul/well) in conjunction with PI8437-01X as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human IL-15. Western Blot: 0.1-0.2ug/ml. The detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS containing 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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