Source: Two DNA sequences encoding the mature mouse IL-17A (Thr 22-Ala 158, Accession Q62386) and mature mouse IL-17F (Arg 21-Ala 153, Accession AAM77568) were expressed separately in E. coli. Molecular Mass: The recombinant mouse IL1-7A/IL-17F is a refolded disulfide-linked heterodimer of IL-17A and IL-17F. The non-methionyl form of rhIL-17A monomer has a predicted molecular mass of 14.9kD whereas the methionyl form of rhIL1-7F monomer has a predicted molecular mass of 15.5kD. Activity: Measured by its ability to stimulate induce IL-6 production by NIH/3T3 cells (Yao, Z. et al., 1995, Immunity 3:811). The ED 50 for this effect is typically 0.075-0.375ug/ml. Endotoxin: 1.0 EU/1ug (LAL) Storage and Stability: 12 months from date of receipt, 20 to 70C as supplied. 1 month, 2 to 8C under sterile conditions after reconstitution. 3 months, 20 to 70C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
95% by SDSPAGE under reducing conditions and visualized by silver stain.
Form:
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in 30% acetonitrile, 0.1% TFA, 50ug of bBSA per 1ug of cytokine. Reconstitute with sterile 4mM HCI containing at least 0.1% HSA or BSA be added to the vial to prepare a stock solution of
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