Interleukin 22, Recombinant, Human (IL-22, CRF2-9, IL-TIF R1, IL-10 Related T Cell-derived Inducible Factor)
Biozol Catalog Number:
USB-I8443-13A
Supplier Catalog Number:
I8443-13A
Alternative Catalog Number:
USB-I8443-13A-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Human IL-22 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179aa residue protein with a putative 33aa signal peptide that is cleaved to generate a 147aa mature protein that shares ~79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIFa and IL-TIFb, share > 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Ra (previously known as CRF2-4), belonging to the class II cytokine receptor family. E. coli-derived human IL-22 protein, Ala34-Ile179, with an N-terminal Met Molecular Mass: The 147aa methionyl form of recombinant human IL-22 has a predicted Mr ~16.5kD. Amino Acid Sequence: MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI Activity: Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is 60-300pg/mL. Nagalakshmi, M.L. et al. (2004) International Immunopharmacology 4:679 Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Stable for 12 months at -20C or colder. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20C or colder. Reconstituted product is stable for 3 months at -20C or colder. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
