Interleukin 22, Recombinant, Human (IL-22, Interleukin-22, IL-TIF, Cytokine ZCYTO18)

Product Overview

• Article Name: Interleukin 22, Recombinant, Human (IL-22, Interleukin-22, IL-TIF, Cytokine ZCYTO18)
• Biozol Catalog Number: USB-I8443-13C
• Supplier Catalog Number: I8443-13C
• Alternative Catalog Number: USB-I8443-13C-2,USB-I8443-13C-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IL-22 is a member of the IL-10 family of regulatory cytokines, which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10Rbeta/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family. Recombinant human IL-22 is a 33.6kD, non-disulfide-linked, homodimeric protein consisting of two 147 amino acid polypeptide chains. Recombinant protein corresponding to human IL-22 expressed in E. coli. Swiss/Uniprot: Q9GZX6 Molecular Weight: ~33.6kD Amino Acid Sequence: MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI Biological Activity: Assay 1: Determined by its ability to stimulate the proliferation of HEK-Blue(TM) IL-22 reporter cells. Assay 2: Measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells). The expected ED50 for this effect is 0.7- 1ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 33.6
• UniProt: Q9GZX6
• Purity: 98% (SDS-PAGE, HPLC). Endotoxin: 0.1ng/ug (1EU/ug)
• Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml. For extended storage, the addition of 0.1% BSA is recommended. Do not vortex.