| Ionomycin was isolated from Streptomyces conglobatus as a potent antibiotic selective for Gram +ve bacteria. During the isolation it was recognised that ionomycin exhibits a very high affinity and selectivity for calcium ions suggesting the metabolite acts as a calcium ionophore. More recently, ionomycin has been used in cell biology as a universal calcium ionophore to explore the role of calcium regulation in the cell. Synonym: (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2R,5S,5S)-octahydro-5-[(1R)-1-hydroxyethyl]-2,5-dimethyl[2,2-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid Source: Streptomyces conglobatus CAS No: 56092-81-0 Molecular Formula: C41H72O9 Molecular Weight: ~709kD Purity: 98% (HPLC) Appearance: Supplied as a solution in Ethanol Solubility: Soluble in Ethanol, Methanol, DMF, DMSO. Poor water solubility. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
