Recombinant protein corresponding to Met1-Ser693 from human IRAK1, expressed in E. coli.
IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that regulate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. All IRAKs are multidomain proteins, containing a conserved N-terminal death domain as well as a central kinase domain that is only active in IRAK1 and IRAK4. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml shows lysates of K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. Reducing conditions. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
