Peptide corresponding to aa546-564 of human IRAK2.
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor 1 (IL-1R1) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation. Members in the IRAK/Pelle family play a central role in IL-1R- and TLR-mediated inflammatory response. IRAK2 is expressed in a variety of human tissues. Applications: Suitable for use in ELISA, Western Blot., Immunohistochemistry Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:500 (a 65kD band should be detected) Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from HeLa or K562 cells Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, 0.01% sodium azide, 40% glycerol.
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