JNK1 alpha1, Active, Recombinant, Human, His-Tag (c-Jun N-terminal Kinase 1, JNK, JNK-46, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated

Product Overview

• Article Name: JNK1 alpha1, Active, Recombinant, Human, His-Tag (c-Jun N-terminal Kinase 1, JNK, JNK-46, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated
• Biozol Catalog Number: USB-J2500
• Supplier Catalog Number: J2500
• Alternative Catalog Number: USB-J2500-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Recombinant protein corresponding to full-length human JNK1a1/SAPK1c with an N-terminal His-tag, expressed in Sf21 cells. Specific Activity: 39u/mg where one Unit of JNK1a1/SPAK1c activity is equal to 1nmol phosphate incorporated into GST-ATF2 per minute. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 43
• UniProt: P45983
• Purity: 90% using Ni-NTA agarose. Mr ~43kD. Purity was assessed by SDS-PAGE and Coomassie blue staining using 2.5ug of active JNK1a1.
• Form: Supplied as a frozen solution in 50mM Tris-HCl, pH 7.5, 270mM sucrose, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF.