The Jo-1 antigen is histidyl-transfer ribonucleic acid (t-RNA) synthetase. This enzyme is partially responsible for attaching t-RNA to their cognate rRNA. The Jo-1 antigen migrates as a 53kD protein on SDS-PAGE. Source: Rabbit thymus Concentration: ~0.43mg/ml Unit Definition: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions/protocols. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography. Other antigens (Sm, RNP, SSA, Scl-70, SSB) are not detected with ELISA assays.
Form:
Supplied as a liquid in 0.01M Tris, 0.53M sodium chloride, 0.02% sodium azide, 50% glycerol.
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