Kallikrein 11, Recombinant, Human (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)
Biozol Catalog Number:
USB-K0005-14D
Supplier Catalog Number:
K0005-14D
Alternative Catalog Number:
USB-K0005-14D-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate. rhKLK11 corresponds to isoform 1. Source: Human CD33 Signal Peptide (Met 1-Ala 16) Human Kallikrein 11 (Glu 19-Asn 250) HHHHHHHHHHNC Recombinant human Kallikrein 11 (rhKLK11, residues 19-250)1-4 in the above construct was expressed in a murine myeloma cell line, NS0. Molecular Mass: The purified, secreted rhKLK11 corresponds to the pro form with an N-terminal sequence of E19TRIIKGFE. By SDS-PAGE under reducing conditions, the apparent molecular mass of the pro form is approximately 40kD. Endotoxin: 1EU per 1ug of the protein as determined by the LAL method. Use: To activate rhKLK11, pre-incubate it at 100ug/ml with thermolysin at 10ug/ml in 50mM Tris,10 mM CaCl2, 0.15M NaCl, pH 7.5 (TCN) for 15 min at 37 C. Add EDTA (10mM final concentration) to stop thermolysin activity. Specific activity: Measured with 100uM substrate and 100ng enzyme in 100ul of 50mM Tris, 1M NaCl, 10mM EDTA, pH 8.5, 0.1mM DTNB at RT, is 450pmoles/min/ug. Activity can be inhibited by AEBSF, dichloroisocoumarin and aprotinin. Shipping: The protein is shipped with ice packs. Samples are stable for up to twelve months at -70C. This enzyme can be aliquoted and stored under sterile conditions at -20 C to -70 C in a manual defrost freezer for three months without a significant loss of activity. Avoid repeated freeze-thaw cycles.
