Ketone Body Assay Kit, BioAssay(TM)

Product Overview

• Article Name: Ketone Body Assay Kit, BioAssay(TM)
• Biozol Catalog Number: USB-K0551-50
• Supplier Catalog Number: K0551-50
• Alternative Catalog Number: USB-K0551-50-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Ketone Bodies (acetoacetic acid and 3-hydroxybutyric acid) are produced in the liver mainly from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypo-glycaemia, growth hormone deficiency, alcohol or salicylate intoxication and inborn errors of metabolism. Simple, direct and automation-ready procedures for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH) are very desirable. Ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations, Applications: Direct assays of ketone body in serum, plasma, urine and other biological samples. Key Features: Sensitive and accurate. Uses 10ul sample. Linear detection range of 0.12 to 8mM for each ketone body in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature. High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day. Kit Components (200 Tests in 96 well plate): K0551-50A: AcAc Buffer, 1x20ml *K0551-50B: AcAc Reagent,1x1 vial K0551-50C: AcAc Standard, 1x200ul K0551-50D: HBDH Enzyme, 1x120ul K0551-50E: BOH Buffer, 1x20ml BK0551-50F: BOH Reagent, 1x1ml K0551-50G: BOH Standard, 1x200ul Storage and Stability: Store all components at -20C. After reconstitution *K0551-50B is stable for 3 weeks. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.