The three Ig-domain from of inhibitory killer cell Ig-like receptor 1 (KIR3DL1, NKB1, nkat3, p70KIR) is a NK cell receptor for polymorphic HLA-B determinant. KIR3DLl recognizes the Bw4 determinant defined by sequence motifs at positins 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR, which contains two immunoreceptor tyrosine-based inhibition motifs (ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity. His-tag fusion protein corresponding to from human KIR3DL1 cytoplasmic tail, overexpressed as insoluble protein aggregates (inclusion bodies), expressed in E. coli. Molecular Weight: ~15kD (132 amino acids) Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHL WCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
15
Purity:
95% by SDS PAGE, Purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
Form:
Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride.
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