D-Lactate Assay Kit, BioAssay(TM), Colorimetric

Product Overview

• Article Name: D-Lactate Assay Kit, BioAssay(TM), Colorimetric
• Biozol Catalog Number: USB-L1011-04
• Supplier Catalog Number: L1011-04
• Alternative Catalog Number: USB-L1011-04-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565nm, is proportionate to the lactate concentration in the sample. Key Features: Sensitive and accurate: The detection limit of 0.05mM and linearity up to 2mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1mM and linearity up to 1mM D-lactate in a 96-well plate assay. Convenient: The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 minutes. Room temperature assay. No 37C heater is needed. High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Application: Direct Assays: D-lactate in serum, plasma, and cell media samples. Kit Contents: (100 tests in 96-well plates): L1011-04-A: Assay Buffer, 1x10ml L1011-04-B: Enzyme A, 1x120ul L1011-04-C: Enzyme B, 1x120ul L1011-04-D: NAD Solution, 1x1ml L1011-04-E: MTT Solution, 1x1.5ml L1011-04-F: Standard (20mM D-Lactate), 1x1ml Storage and Stability: Store components at -20C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.