Lactate Dehydrogenase B, Recombinant (LDHB, LDH-B, L-Lactate dehydrogenase B chain)
Biozol Catalog Number:
USB-L1011-08
Supplier Catalog Number:
L1011-08
Alternative Catalog Number:
USB-L1011-08-3
Manufacturer:
US Biological
Category:
Molekularbiologie
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of lactate and pyruvate. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. Recombinant LDH-B was produced using LDH-B DNA cloned from cDNA library of chicken heart. Biological Activity: ~258 U/mg protein Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95% by RP-HPLC and SDS-PAGE. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
Form:
Supplied as a lyophilized powder in potassium phosphate. Reconstitute with sterile ddH2O to 100ug/ml, which can then be further diluted to other aqueous solutions.
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