Lens culinaris agglutinin has a molecular weight of approximately 49,000 and is composed of four subunits-two of about 17,000D and two of 8,000D. The isoelectric point of LCA is approximately pH 8.5. LCA recognizes sequences containing a-linked mannose residues. Like other mannose specific lectins, divalent cations such as calcium and manganese are required for sugar binding activity. By recognizing additional sugars as part of the receptor structure, LCA has a narrower specificity than Con A. For example, an a-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide markedly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A. LCA has been employed to separate lymphocyte populations, as a potent T-cell mitogen, and as one of the most effective agents in preventing skin allograft rejection in model systems. LCA is also used to purify numerous glycoproteins (including immunoglobulins, histocompatibility antigens, a2-macroglobulin, etc.) as well as to fractionate glycopeptides from a variety of glycoproteins. Fluorescein labeled Lens Culinaris Agglutinin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Lens Culinaris Agglutinin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Applications: Suitable for use in FLISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Excitation: 495nm Emission: 515nm F/P (molar): As Reported Sugar Specificity: Mannose, Glucose Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form:
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide, 0.1mM Ca++, 0.01mM Mn++. Labeled with FITC.
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