Leptin is a 16kD nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Molecular Weight: ~16kD (147 amino acids) Amino Acid Sequence: MVPIQKVQDD/ TKTLIKTIVT/ RINDISHTQS/ VSAKQRVTGL/ DFIPGLHPIL/ SLSKMDQTLA/ VYQQVLTSLP/ SQNVLQIAND/ LENLRDLLHL/ LAFSKSCSLP/ QTSGLQKPES/ LDGVLEASLY/ STEVVALSRL/ QGSLQDILQQ/ LDVSPEC Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
16
Purity:
95% by SDS PAGE
Form:
Supplied as a liquid in PBS, pH 7.4
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