Leptin, Recombinant, Human (Ob, Obese Protein, Obesity Factor)
Biozol Catalog Number:
USB-L1670-15J
Supplier Catalog Number:
L1670-15J
Alternative Catalog Number:
USB-L1670-15J-500
Manufacturer:
US Biological
Category:
Molekularbiologie
Human leptin is a 16kD nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation. Recombinant human leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Molecular Weight: 16kD (147amino acids) Sequence: MVPIQKVQDD/ TKTLIKTIVT/ RINDISHTQS/ VSSKQKVTGL/ DFIPGLHPIL/ TLSKMDQTLA/ VYQQILTSMP/ SRNVIQISND/ LENLRDLLHV/ LAFSKSCHLP/ WASGLETLDS/ LGGVLEASGY/ STEVVALSRL/ QGSLQDMLWQ/ LDLSPGC Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
16
Purity:
95% by SDS PAGE
Form:
Supplied as a liquid in PBS, pH 7.4
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