Leptin, Recombinant, Rat (Obese Protein, Obesity Factor)
Catalog Number:
USB-L1670-16
- Images (0)
| Article Name: | Leptin, Recombinant, Rat (Obese Protein, Obesity Factor) |
| Biozol Catalog Number: | USB-L1670-16 |
| Supplier Catalog Number: | L1670-16 |
| Alternative Catalog Number: | USB-L1670-16-200,USB-L1670-16-1 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant protein corresponding to rat Leptin containing 147 amino acid residues, expressed in E. coli. Uniprot/Swiss Accession: P50596 Amino Acid Sequence: MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC Biological Activity: Determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5µg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile sterile dH2O with 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Product Guarantee and Expert Support
