Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Recently, Ob genes (mouse and human) have been cloned. Obese gene encodes an adipocyte-tissue derived secreted Ob protein/Leptin (167aa ~16kD) that controls body weight homeostasis. Exogenous administration of recombinant Ob protein can reduce food intake and body weight. However, Ob protein had no effect in db/db mice suggesting a defect in leptin signaling mechanism. The Obese receptors (Ob-R) have now been cloned from mouse choroid plexus and it is expressed in several tissues including hypothalamus. The Ob-R has been shown to be a product of db gene that has long been thought to encode the receptor for a weight-controlling hormone. The Ob-R has at least 6 alternatively spliced forms with modifications at the amino and C-terminus. The Ob-R varies in length after Lysine889. The Ob-Ra represents the initially identified mouse Ob-R (short form, 894aa). Ob-Rb displays ~78% homology to the human Ob-R (long form, 1165aa). Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice and missing the cytoplasmic domain thought to be important for leptin signaling. Purified human Leptin (1-10ug/gm of body weight, daily i.p injections for 14 days) has been shown to be biologically active in reducing body weight and food consumption in ob/ob and NZO mice. Source: Recombinant ovine leptin (146aa), cloned, expressed in E. coli. Molecular Weight: ~16.13kD Biological Activity: Recombinant purified ovine leptin protein has ED50, calculated by the leptin-dependent stimulation of Human OB-R transfected murine BaF3 indicator cells is 0.33-0.05ng/ml. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
16.13
Purity:
~95% (SDS-PAGE). Endotoxin: ~10pg/ug
Form:
Supplied as a liquid in isotonic PBS.
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