LOXL2, Recombinant, Human (Lysyl Oxidase-like Protein 2, Lysyl Oxidase Related Protein 2, LOR2, Lysyl Oxidase Homolog 2, Lysyl Oxidase Related Protein WS9-14)

Product Overview

• Article Name: LOXL2, Recombinant, Human (Lysyl Oxidase-like Protein 2, Lysyl Oxidase Related Protein 2, LOR2, Lysyl Oxidase Homolog 2, Lysyl Oxidase Related Protein WS9-14)
• Biozol Catalog Number: USB-L2623-15
• Supplier Catalog Number: L2623-15
• Alternative Catalog Number: USB-L2623-15-25
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Source: Recombinant corresponding to aa500-end of human LOXL2 expressed in a baculovirus-infected Sf9 cell expression system (NM_002318). Activity: Enzyme activity is assayed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL-2 (1-2 ng/ul) is incubated with 3mM 1,5-diaminopentane, 0.4mM AmplexRed and 0.2unit/ul peroxidase in 100ml of the assay buffer (1.2M Urea, 50mM borate, pH 8.8) for 30 min at 30C. Fluorescence intensity is measured every 7 minute at 530ex/590em. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70C. Aliquots are stable for at least 6 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 32
• NCBI: 002318
• Purity: Purified ~65%
• Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.