Luteinizing Hormone Releasing Hormone, Human, Control Peptide (LHRH)
Biozol Catalog Number:
USB-L7510-02B
Supplier Catalog Number:
L7510-02B
Alternative Catalog Number:
USB-L7510-02B-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Human reproduction is regulated by Luteinizing hormone releasing hormone (LHRH), also termed gonadotropin releasing hormone (GnRH). It is produced by hypothalamic neurons, secreted in a pulsatile manner into the capillary plexus of the median eminence and effects the release of luteinizing hormone and follicle stimulating hormone from gonadotropic cells in the anterior pituitary. The peptide may also influences other activities including behavioral ones, as LHRH or LHRH-like immunoreactivity has been found in gonadal tissue, placenta and the central nervous system, and exogenously administered LHRH is shown to affect behavior. LHRH gene (chromosome 8q21) code for a protein of 92 amino acids (rat 92 aa and mouse 90 aa) in which the LHRH decapeptide is preceded by a signal peptide of 23 amino acids and followed by a Gly-Lys-Arg sequence, as expected for enzymatic cleavage of the decapeptide from its precursor and amidation of the carboxy-terminal of LHRH. In addition to the LHRH decapeptide, the prohormone contains a 56-amino acid peptide sequence which has been designated gonadotropin-releasing hormone associated peptide (GAP). Source: Human LHRH (pGlu HW SYG LRP G) (1) Species Sequence Homology: Mouse, rat-100%. Applications: Suitable for use in ELISA as a control peptide for L7510-02 & L7510-02A. Other applications not tested. Recommended Dilution: ELISA: Coat ELISA plates at 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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