Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, macrophages and endothelial cells. This colonly stimulating factor is involved in both the development and function of monocytes/macropahges. Human M-CSF is a disulfide-linked homodimer composed of 18.4kD subunits of 158 amino acid residues. Amino Acid Seqeunce: MEEVSEYCS HMIGSGHLQ SLQRLIDSQ METSCQITF EFVDQEQLK DPVCYLKKA FLLVQDIME DTMRFRDNT PNAIAIVQL QELSLRLKS CFTKDYEEH DKACVRTFY ETPLQLLEK VKNVFNETK NLLDKDWNI FSKNCNNSF AECSSQGHE RQSEGS Biological Activity: Human M-CSF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is 1ng/ml, corresponding to a specific activity of 1x10e6 units/mg. . Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
36.8
Purity:
98% (SDS-PAGE and HPLC analyses). Endotoxin: 0.1ng/ug (1EU/ug).
Form:
Supplied as a lyophilized powder from 5mM Tris, pH 8.0, with 75mM sodium chloride. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Do not vortex. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1% BSA.
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