MAP Kinase 1, Recombinant Human, His-Tag, aa1-360 (Mitogen-activated Protein Kinase 1, MAPK 1, MAPK1, ERT1, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERK, MAP Kinase Isoform p42, Mitogen-activated Protein Kinase 2, MAP

Product Overview

• Article Name: MAP Kinase 1, Recombinant Human, His-Tag, aa1-360 (Mitogen-activated Protein Kinase 1, MAPK 1, MAPK1, ERT1, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERK, MAP Kinase Isoform p42, Mitogen-activated Protein Kinase 2, MAP
• Biozol Catalog Number: USB-M2352-03D
• Supplier Catalog Number: M2352-03D
• Alternative Catalog Number: USB-M2352-03D-100,USB-M2352-03D-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

MAPK1, also known as ERK (extracellular signal-regulated kinase), acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Recombinant human MAPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa1-360 frin human MAPK1, fused to His-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot Accession: P28482/NP_620407 Molecular Weight: ~43.5kD (380aa) confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 43.5
• NCBI: 620407
• UniProt: P28482
• Purity: 95% (SDS-PAGE) Purified by immunoaffinity chromatography.
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.