p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY*, resulting in p38 activation. Sections are cut freshly upon ordering. Description: Each control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, both untreated and treated with Phorbol-13-Myristate-12-Acetate (PMA), that serve as a control for Phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. PMA induces phosphorylation of p44/42 MAPK. Western Blot analysis was performed on extracts derived from the same cells to verify the efficacy of the the PMA treatment. Applications: These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides. Companion Products Phospho-p44/42 MAPK (Thr202/Tyr204) Rabbit mAb (IHC Preferred) M2352-31: Growth/Proliferation Marker: SignalStain Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Detection Kit Phospho-p44/42 MAPK (Thr202/Tyr204) Blocking Peptide (IHC Specific) p44/42 MAP Kinase Rabbit mAb p44/42 MAP Kinase Blocking Peptide p44/42 MAP Kinase (L34F12) Mouse mAb p44/42 MAP Kinase Blocking Peptide M2360-02: p44/42 MAP Kinase Antibody M2352-11A: p42 MAP Kinase (Erk2) Antibody Storage: Store at 4C. Optimal staining is achieved if slides are stained following our standard IHC protocols and are used within 8 weeks of assay date, however, signals may persist beyond two months.
