Recombinant protein corresponding to human MART-1. Cellular Localization: Cytoplasmic
MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein A. Western Blot: 0.5-1ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. M2410-12 is preferred for this application. Optimal dilutions to be determined by the researcher. Positive Control: CaC1 melanoma cells and melanomas Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[0.N.396 M2-7C10]
Purity:
Purified by Protein A affinity chromatography from ascites.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
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