Maspin, Recombinant, Human (Mammary-specific Serpin, SERPINB5, Protease inhibitor 5)
Biozol Catalog Number:
USB-M2412-50C
Supplier Catalog Number:
M2412-50C
Alternative Catalog Number:
USB-M2412-50C-5,USB-M2412-50C-20
Manufacturer:
US Biological
Category:
Molekularbiologie
Maspin or mammary-specific serpin is a tumor suppressor protein of serine proteinase inhibitor family. Its transcription is regulated in combinatorial fashion by Ets, p53 and AP-1. It is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas. The extent of downregulation correlates with the progression of the carcinoma and may help in prognosis. Maspin has the ability to block the growth, invasiveness, and metastatic potential of breast and lung tumors. This anti-tumor activity is achieved, in part, by the ability of Maspin to inhibit angiogenesis and to preferentially promote apoptosis of tumor cells. Recombinant human Maspin is a 42.2kD non-glycosylated protein containing 375 amino acid residues. Amino Acid Sequence: MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, carrier protein. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
