Recombinant protein corresponding to Gly20-Asn247 from human Chymase expressed in NS0 cells. Swiss/UniProt Accession: P23946.
Chymase (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa22-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa49-247). Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using lysate of human small intestine tissue Immunoprecipitation: 25ug/ml using rhChymase from conditioned media of transfected NS0 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
