Recombinant GST-mouse mMCP-8 fusion protein expressed in E. coli.
mMCP-8 and its rat homologues, rMCP-8, -9, and -10, form a new group of mast cell/basophil proteases, which are more closely related to the T-cell granzymes and neutrophil cathepsin G than to the mast cell tryptases and chymases. mMCP-8 showed a high degree of homology with mouse granzyme B in the critical regions for determining substrate cleavage specificity. It preferentially cleaves after Asp residues. mMCP-8 mRNA is highly expressed in a mouse connective tissue MC-like tumor line. However, it could not be detected in mouse liver, intestine, lung or ears, indicating very low expression in normal tissues. A strong increase in mMCP-8 levels in the lungs can be detected in infected animals. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[TUG8]
Isotype:
IgG2a
Purity:
Purified by affinity chromatography
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
* VAT and and shipping costs not included. Errors and price changes excepted
