Matriptase, also called MT-SP1, TADG-15 and ST14, is a member of the type II transmembrane serine protease family and is expressed on most human epithelia as an 80-90kD acell surface glycoprotein. Processing, activation and shedding of the C-terminal 26kD peptidase S1 domain is complex and requires coexpression with its inhibitor, HAI-1. Matriptase activity is critical to epidermal barrier formation. It is overexpressed in a variety carcinomas. Human and mouse matriptase share 86% aa identity within the peptidase S1 domain. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:1000 Immunofluorescence: 1:100-1:500 Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
