Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. (1995) as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22. Applications: Suitable for use in Western Blot an immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[10K273]
Isotype:
IgG1
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
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