Human macrophage/monocyte chemotactic protein-4 (MCP-4) is a member of the b-chemokine family (C-C) of cytokines. Like eotaxin, MCP-4 is a powerful eosinophil chemoattractant. Additionally, like MCP-3, MCP-4 elicits chemoattraction of monocytes and T lymphocytes. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: ELISA: 1-2ug/ml (100ul/well). Use as capture antibody in sandwich ELISA. Neutralization: 1-5ug/ml Optimal dilutions to be determined by the researcher Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[4F2 G7_89.3]
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
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