A 375P cells crude extract. Cellular Localization: Cell membrane
Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. MCSP is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300kD and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumors. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:400 Western Blot: 1:1000 Immunofluorescence (IC): 1:100 Immunoprecipitation: 1:10-1:100 Immunohistochemistry(Paraffin/frozen): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
