MDA5 (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1), Rabbit
Biozol Catalog Number:
USB-M2772-75
Supplier Catalog Number:
M2772-75
Alternative Catalog Number:
USB-M2772-75-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
WB
Immunogen:
16 amino acid peptide from near the center of human MDA5 (GenBank accession no. NP_071451)
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses (1,2). Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family (3), consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kappaB (4). MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: M2772-75A: MDA5, Human, Control Peptide (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1) Recommended Positive Control: Daudi cell lysate Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
