MDA5 (Melanoma Differentiation-Associated Protein 5, DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide, Helicard, Hlcd, Helicase with 2 CARD Domains, IDDM19, Interferon-induced with Helicase C Domain Protein 1, Interferon-induced Helic
Biozol Catalog Number:
USB-M2772-75H
Supplier Catalog Number:
M2772-75H
Alternative Catalog Number:
USB-M2772-75H-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
ELISA
Immunogen:
Synthetic peptide corresponding to C-KQKLRNNDLKKQN, from the internal region of mouse MDA5/IFIH1.
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses . Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kappaB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity. Applications: Suitable for use in Peptide ELISA. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:16,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
