b2-Microglobulin, Human Western Blot Control (b2M)

Product Overview

• Article Name: b2-Microglobulin, Human Western Blot Control (b2M)
• Biozol Catalog Number: USB-M3890-55B
• Supplier Catalog Number: M3890-55B
• Alternative Catalog Number: USB-M3890-55B-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: WB

Product Description

Positive control for Beta-2-microglobulin M3890-55 and M3890-55A. Purified human B2M protein is formulated in SDS-PAGE sample buffer (reduced) to be used as a positive control for various anti-B2M antibodies using Western Blot. It is ready to be loaded on gels after heating. Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119 aa, chromosome 15). B2M associates with heavy hcain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene. Applications: Suitable for use in Western Blot only. Recommended Dilutions: Western Blot: 10ul/lane Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.