Source: Pooled normal canine milk Applications: Suitable for use as a qualitative reference with an optimized protein content to study the normal protein composition of canine milk. Can be used to identify or quantitate a normal milk protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. Works as a reference in nephelometry and other automated precipitation techniques. As a blocking agent or as a negative control in non-precipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. Can also be used as an internal relative standard for quantitative protein assays such as double radial immunodiffusion (Mancini, Fahey), ELISA, Western Blot and electroimmunodiffusion (Laurell), expressing the results obtained with the sample under investigation as a percentage of the protein concentration in the normal reference milk. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form:
Supplied as a lyophilized powder. Preservative free. Reconstitute with 500ul sterile dH2O using inversion, mixing gently. Allow to stand 5-10 minutes at RT.
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