Description: Pooled, delipidated, heat-inactivated, lyophilized human whole milk. Applications: Suitable for use as a qualitative reference with an optimized protein content to study the normal protein composition of human milk. To identify or quantitate a normal milk protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. As a reference in nephelometry and other automated precipitation techniques. As a blocking agent or as a negative control in non-precipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. This normal reference does not contain assigned values for individual proteins but can be used as an internal relative standard for quantitative protein assays such as double radial immunodiffusion (Mancini, Fahey), ELISA, Western Blot and electroimmunodiffusion (Laurell), expressing the results obtained with the sample under investigation as a percentage of the protein concentration in the normal reference milk. Storage and Stability: Lyophilized powder may be stored at -20C and is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. To reconstitute, add 0.5ml sterile dH2O and mix gently by inversion until complete dissolution is obtained. Allow ot stand at room temperature for 5-10 minutes to reach equilibrium. Reconstituted milk may be stored frozen.
Form:
Supplied as a lyophilized powder. Preservative free.
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