Lactadherin is a widely distributed glycoprotein (~ 50kD), which was originally characterized due to its association with milk fat/lipid globule membranes. Synonymous names are PAS-6/7, bovine-associated mucoprotein, BA-46, P47, and MFG-E8. Structural hallmarks of lactadherin are the presence of two epidermal growth factor (EGF) homology domains (with an RGD peptide motif in the second EGF domain), and two C domains sharing homology with the discoidin family of lectin domains including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin shows preferential binding to phosphatidylserine (L-form) in a calcium independent manner. Purified lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins (10). Fluoresence-labeled lactadherin functions as a sensitive probe for exposed phosphatidylserine on nucleated cells and on stimulated platelets (8, 9). Lactadherin will bind to membranes that have phosphatidylserine content below the threshold for annexin V binding. Localization: Milk (1), also secreted by stimulated macrophages (2), vascular smooth muscle (3), endothelial cells(4) Plasma Concentration: Unknown in normal adults, measurable in women with metastatic breast cancer (5), measurable in newborn calves after milk meal (1). Molecular Weight: 52kD and 47kD (glycosylation variants, PAS-6 and PAS-7 respectively) Extinction Coefficient: E1%, 1cm, 280nm = 16.5 Structure: Single chain with two EGF domains and two C domains. The mouse isoform contains a central proline rich region and the human molecule contains a single EGF domain Percent Carbohydrate: 6%, 13% for glycosylation variants Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Molecular Weight:
52000
Purity:
~95% (SDS-PAGE)
Form:
Supplied as a liquid TBS, pH 7.4, 1% BSA, 0.02% sodium azide. Labeled with FITC. Flourochrome/protein ratio: 1.8/molecule.
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