MluI

Product Overview

• Article Name: MluI
• Biozol Catalog Number: USB-M4140
• Supplier Catalog Number: M4140
• Alternative Catalog Number: USB-M4140-1000,USB-M4140-5000
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-A C G C G T-3 3-T G C G C A-5 Source: Microcccus luteus Concentration: ~10u/ul Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer. Incubation Temperature: 37C Diluent Buffer: 10mM Tris-HCl, pH 7.4 at 25C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol, or Storage Buffer. Storage Buffer: 10mM Tris-HCl, pH 7.5 at 25C, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Supplied With: R1625: Restriction Enzyme Buffer A, 10X R1625-04: Restriction Enzyme Buffer E, 10X Enzyme Properties: Methylation Effects: Dam: Never overlaps - no effect. Dcm: Never overlaps - no effect. CpG: Completely overlaps- blocked. EcoKl: May overlap - no effect. EcoBl: May overlap - no effect. Stability during Prolonged Incubation: A minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20 minutes. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: AflIII, AscI, DsaI, PauI Number of Recognition Sites in DNA: Lambda: 7 PhiX174: 2 M13mp18/19: 0 pBR322: 0 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with MluI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with MluI, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.07uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.