Monocarboxylate Transporter 2, Rat, Control Peptide (MCT2)
Biozol Catalog Number:
USB-M4470-11D
Supplier Catalog Number:
M4470-11D
Alternative Catalog Number:
USB-M4470-11D-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT2/MOT2 (mouse 484 aa, rat 489 aa, human 478 aa, chromosome 12q13) is less widely distributed than MCT. It is associated with tissues that demonstrate a high uptake affinity for lactate and pyruvate such as the kidney and liver (for gluconeogenesis) and neurons (for oxidation). It may function in transporting lactate from intestine and erythrocytes. Source: A 15aa peptide sequence within the cytoplasmic, C-terminus of rat MCT2 Applications: Suitable for use in ELISA. Because of its low mol. Wt (<3kD), is not suitable for Western. Other applications not tested. Recommended Dilution: ELISA: 1:10K-1:100K, using 50-100 ng of control peptide/well. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly Purified
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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