Monocarboxylate Transporter 4, Human, Control Peptide (MCT4)
Biozol Catalog Number:
USB-M4470-30F
Supplier Catalog Number:
M4470-30F
Alternative Catalog Number:
USB-M4470-30F-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Control peptide for M4470-30 (affinity purified antibody) and M4470-30D (antiserum). Synthetic peptide consisting of 19aa sequence within the cytoplasmic loop 4 of human MCT4. No significant sequence homology exists with other MCTs. Species Sequence Homology: Mouse MCT4: 83%. Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT4/MOT4/SLC16A3 (mouse 470aa, rat 471aa, human 465-a, chromosome 17q25) is most closely related to MCT3, is prominently expressed in skeletal muscle and other cells with a high glycolytic rate such as tumor cells and white blood cells, suggesting an important role in lactic acid efflux. According to new nomenclature, the old MCT3 has now been reclassified as MCT4. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: ELISA: 50-100ng of control peptide/well. Antibody Blocking: 5-10ug per 1ug M4470-30 (affinity purified antibody) or per 1ul M4470-30D (antiserum). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
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