Metal Transporter Protein, Mouse, Control Peptide (MTP1)

Product Overview

• Article Name: Metal Transporter Protein, Mouse, Control Peptide (MTP1)
• Biozol Catalog Number: USB-M4695-50A
• Supplier Catalog Number: M4695-50A
• Alternative Catalog Number: USB-M4695-50A-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA

Product Description

Control Peptide for M4695-50 (antiserum) and M4695-50B (affinity purified antibody). Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A number of ferric reductases have been identified in yeast, plants and bacteria. The identity of such proteins remained unknown in the mammals. Most recently, a new gene called Dcytb (for duodenal cytochrome b) has been cloned and characterized in mouse duodenum that may function as mammalian ferric reductase. A novel iron-regulated gene that is homologous to the DMT1, termed Metal Transporter Protein (MTP1, human 571aa, mouse 570aa, ~90% similarity), has been identified. MTP1 is also described as iron-regulated transporter (IREG1) and Ferroportin1. MTP1 contains IRE in the 5-UTR. It is expressed in tissues involved in body iron homeostasis including the developing and mature reticuloendothelial system, the duodenum and the pregnant uterus. MTP1 is also expressed in muscle and embryonic brain. In the adult mouse, MTP1 expression in the liver and duodenum are reciprocally regulated. Iron deficiency induces MTP1 expression in the duodenum and down-regulates expression in the liver. MTP1 may be involved in intracellular iron metabolism. Applications: Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml to coat ELISA plates. Antibody Blocking: 5-10ug per 1ug M4695-50B (affinity purified antibody) or per 1ul M4695-50 (antiserum). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Highly purified
• Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.