NCX3, Rat, Control Peptide (Sodium Calcium Exchanger)

Product Overview

• Article Name: NCX3, Rat, Control Peptide (Sodium Calcium Exchanger)
• Biozol Catalog Number: USB-N0569-30
• Supplier Catalog Number: N0569-30
• Alternative Catalog Number: USB-N0569-30-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA

Product Description

Control peptide for N0569-30 (antiserum) and N0569-35 (affinity purified antibody). Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein. NCX1 (rat 971aa, human 970aa, mouse 970aa) is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling, but is also present in most other tissues. Alternative spicing of NCX1/NACA1 produces numerous tissue specific isoforms (heart NACA1, Kidney NACA2, -3, and -7, brain NACA4-6). NCX2 (rat 921aa) is restricted to brain and skeletal muscle. NCX3 (rat 927aa) shares 73-75% with NCX1 and NCX2. NCX3 is also restricted to brain and muscle. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitabe for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: Antibody Blocking: 5-10ug per 1ul N0569-30 (antiserum) or 1ug N0569-35 (affinity purified antibody). ELISA: 50-100ng/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Highly purified
• Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.