Nectin 3 (Nectin-3, CD113, Poliovirus Receptor-related Protein 3, PRR3, Pvrl3), IgG2a, Clone: [103-A1], Rat, Monoclonal
Biozol Catalog Number:
USB-N0571-55D
Supplier Catalog Number:
N0571-55D
Alternative Catalog Number:
USB-N0571-55D-100
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
ELISA, FC, IHC, IP
Immunogen:
Recombinant corresponding to aa50-400 from the extracellular domain of mouse Nectin 3, fused to IgG Fc.
Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin/PRR-family, also described as the alpha herpes virus receptor family consists of four members, nectin 1, 2, 3 and 4. All the members of the nectin family have two or three slice variants. For nectin 3, nectin 3alpha, 3beta and 3gamma isoforms exist. Both nectin 2 and nectin 3 are ubiquitously expressed whereas nectin 1 is abundantly expressed in brain. Nectin 2 or 3 is expressed in cells where cadherin are not expressed, such as blood cells and spermatids. Nectin 3 is a transmembrane protein that is mainly expressed in testis and placental tissues. Nectin 3/PRR3 interacts in vivo with both long and short isoforms of afadin. Applications: Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Functional Assays and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1ug/1x10e6 cells Immunohistochemistry (Frozen): 1:10 Fixation with PFA or acetone/methanol is recommended. Immunoprecipitation: 1-2ug/100-500ug of total protein (1ml of cell lysate) Indirect ELISA: 0.5ug/ml mouse nectin-3 coated per well. Positive signal with 2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: L-cells (A cultured line of C3H mouse fibroblasts), Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[103-A1]
Isotype:
IgG2a
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
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