Recombinant protein corresponding to Met618-Thr673 from human NLGN-4, expressed in E. coli
Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml NTera-2 human testicular embryonic carcinoma cell line Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human brain (cortex) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.