Control peptide for N2016-03 (antiserum) and N2016-04 (affinity purified antibody). Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Five NHE isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular. NHE2 (rat 813 aa, human chromosome 2 and rat chromosome 9) has been implicated in volume regulation in renal inner medullary collecting duct cells. Its mRNA is found in kidney medulla, cortex, colon, jejunum, ileum, human jejunum, ileum, duodenum, stomach and adrenal glands (1). Applications: Suitable for use in ELISA and antibody blocking experiments. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
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